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#microscopy

11 posts5 participants1 post today
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micronicle LAB

Experimenting with #ThT stain on #spider webs to see if it successfully bonds to the beta chain #fibrin / #amyloid like components. I've applied the stain and naturally under 460nm webs fluoresce the expected green. I'm waiting for the sample to completely dry before differentiating with alcohol and then using a water based mount to finally disassociate the unbounded stain from the webs and other artefacts. All being so, we might be left with ThT stained #spidroins #science #biology #microscopy

10x ThT stained 460nm epi fluorescence
Public
tf

Warty Cavalier (Melanoleuca verrucipes): Usually found in the late summer and autumn, this mushroom was first recorded in the UK only in 2000*, and is mostly found in the south of England. Well, this one appeared in my garden in the Central Belt of Scotland last Friday (11 April)! It popped out in an old abandoned flower pot. ... #Mushrooms, #Fungi, #Nature, #Microscopy: aye.tf/2025/04/14/melanoleuca-

aye, tf · Warty Cavalier (Melanoleuca verrucipes)Usually found in the late summer and autumn, this mushroom was first recorded in the UK only in 2000*, and is mostly found in the south of England. Well, this one appeared in my garden in the Central Belt of Scotland last Friday (11 April)! It popped out in an old abandoned flower pot.
Public
Virgile Andreani

I spent the day rectifying segmentation and tracking for bacteria in microfluidic devices. 1000 frames, 5 chambers, ~8 cells per chamber, so 40000 pictures of cells to look at, and check that they match frame to frame. I think I'm done with screens for the day. Fortunately automation helps, and drawing the lineages as graphs allows to spot errors more easily.
Now I have a clean dataset that I can analyse and also use as a training dataset for the next iteration!

#microscopy#AcademicChatter
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micronicle LAB

I'm what you call a novice microscopist I would say, not beginner or amateur. I'm guessing at what that transparent looking strand with spotted inclusions within it's core, might be found in household dust. Fungal growth?. I have seen threads like this before with trumpet-like joins along it's length, but never saw one quite like this.
Object diameter 1.5um.

Can anyone shed light on the object?

NPL Fluotar 40/0.70 Phaco 2. 5MP CMOS ToupCam.

Leitz Dialux EB, NPL Fluotar 40/0.70 Phaco 2. 5MP CMOS ToupCam.
#biology#mycology#nature
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Moritz Negwer

Here's an interesting way to do photobleaching with cleared tissue: Add 0.1% H2O2 to the clearing medium and blast with LEDs for 24h at RT. Also includes very detailed instructions for building an LED photobleaching setup - the samples ate placed between two PCBs with LEDs in a ventilated box.

Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues
Murakami et al., preprint at biorxiv 2025
biorxiv.org/content/10.1101/20

Figure 5 | Application of the photobleacher to the pigment-rich volumetric tissues after clearing. (A) Schematic illustration of the photobleaching process. The tissues were illuminated after clearing, with hydrogen peroxide included when necessary. (B) Mouse hearts with various photobleaching conditions. Representative single z-planes are shown. (C) Three-dimensional rendering of a mouse heart after IHC staining using an anti-αSMA antibody. (D) Pieces of human cortex from an identical donor, with various photobleaching conditions. Three-dimensional renderings are shown. Outlines of tissues were shown in dot lines when necessary. (E) Three-dimensional rendering of a human cerebral cortex after IHC staining using anti-αSMA and anti-GFAP antibodies.
#tissueclearing#fluorescence#microscopy
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